{"id":168,"date":"2010-02-17T00:43:45","date_gmt":"2010-02-17T00:43:45","guid":{"rendered":"http:\/\/alectos.com\/content\/?p=168"},"modified":"2010-02-17T00:43:45","modified_gmt":"2010-02-17T00:43:45","slug":"visualizing-oga-reaction-coordinate","status":"publish","type":"post","link":"https:\/\/alectos.com\/alectos-content\/index.php\/2010\/02\/17\/visualizing-oga-reaction-coordinate\/","title":{"rendered":"Visualizing the reaction coordinate of an O-GlcNAc hydrolase"},"content":{"rendered":"<p>N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response, and transcriptional regulation. Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) using a substrate-assisted catalytic mechanism. Here we define the reaction coordinate using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.<\/p>\n<p>Source: <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20067256\" target=\"_blank\" rel=\"noopener\">He, Y., Macauley, M.S., Stubbs, K.A., Vocadlo, D.J. &amp; Davies, G.J. <em>J Am Chem Soc<\/em> <strong>132<\/strong>, 1807-9 (2010).<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins  [&#8230;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[19],"tags":[18,20],"class_list":["post-168","post","type-post","status-publish","format-standard","hentry","category-publications","tag-oga","tag-sfu"],"_links":{"self":[{"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/posts\/168","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/comments?post=168"}],"version-history":[{"count":0,"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/posts\/168\/revisions"}],"wp:attachment":[{"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/media?parent=168"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/categories?post=168"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/alectos.com\/alectos-content\/index.php\/wp-json\/wp\/v2\/tags?post=168"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}